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KMID : 0603820170230020128
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Journal of Experimental & Biomedical Science
2017 Volume.23 No. 2 p.128 ~ p.132
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Effects of Streptozotocin, Bisphenol A and Diethylstilbestrol on Production of Reactive Oxygen Species and Lipid Peroxidation in the Boar Sperm
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Lee A-Sung
Lee Sang-Hee
Lee Seung-Hyung
Yang Boo-Keun
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Abstract
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Streptozotocin (STZ), bisphenol A (BPA), and diethylstilbestrol (DES) are known as endocrine disruptors, occurs oxidative stress in animal cells. Generally, oxidative stress induces reactive oxygen species (ROS) and lipid peroxidation of sperm and lead to decreased viability and fertility in pigs. Therefore, we investigated the influence of STZ, BPA and DES on ROS production and lipid peroxidation on boar sperm. Collected sperm were incubated with semen extender containing 10 μM STZ, 10 μM BPA and 20 μM DES for 3, 6 and 9 hours. Intracellular ROS and lipid peroxidation of sperm were analyzed by 2', 7'-dichlorofluorescein diacetate and malondialdehyde methods. The results show that, intracellular ROS was not significantly different among the all treatments, but lipid peroxidation was significantly increased in STZ group at 3 hour after incubation with boar sperm (P<0.05). These results suggest that STZ stimulates lipid peroxidation more than ROS production and may exert a negative effect on sperm fertility.
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KEYWORD
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Streptozotocin , Bisphenol A , Diethylstilbestrol , Reactive oxygen species , Lipid eroxidation , Sperm
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